Developing a chemoenzymatic approach to prepare carbohydrate-based medicines
**Seminars begin at 4:00 PM and will be held in Clark Hall Room 101**
January 20, 2017
Heparin is a hihly sulfate polysaccharide that is widely used as a anticoagulant drug in hospitals. The supply of heparin is currently relying on the isolation from porcine intestines through a long and poorly regulated supply chain. Developing a cost-effective method to prepare synthetic heparin under pharamaceutical grade manufacture facility will improve the safety of heparin drug. However, synthesis of heparin a using a purely chemical approach remains to be challenging. A chemoenzymatic method has been recently developed to prepare synthetic heparin. The methods only involves the use of five different enzymes, including sulfotransferases, an epimerase and glycosyltransferases. Using these enzymes, a highly diverse heparan sulfate oligosaccharide library have been prepared. In addition, this method has been successfully utilized to synthesize ultra-low molecular weight heparin and low-molecular weight heparins, to improve the pharmacology of heparin-based anticoauglant drugs. The enzyme-based synthetic approach has synthesized more than 50 oligosaccharides with different sulfation patterns and size. The availability of hepain-like oligosaccharide library offers a unique tool to study the function and activity relationship of heparin in biological systems.