Isomorphic Fluorescent Nucleosides, Nucleotides and Oligonucleotides

October 28, 2016

Zhenqiang Wang

Nucleic acids experience a variety of perturbations while performing their essential everyday functions. These may include strand cleavage and ligation, local conformational changes, base damage, modification and flipping, as well as structural and environmental perturbations that are induced upon protein and low MW ligand binding. Additionally, nucleosides and nucleotides are involved in numerous biochemical transformations. Isomorphic fluorescent nucleoside analogs, which can serve as faithful surrogates, are powerful probes for investigating nucleic acids structure, dynamics, recognition and damage as well as metabolic processes. The lecture will present the design, synthesis and photophysical properties of new fluorescent isomorphic nucleoside analogs as well as their utilization for the fabrication of "real-time" fluorescence-based discovery and biophysical assays.