Chemical Reporters for Exploring Protein Modifications

March 20, 2015

Howard Hang

Proteins are modified by a variety of metabolites that regulates many important cellular pathways in all kingdoms of life. To facilitate the analysis of protein modifications in biology, metabolite analogues bearing alkyne- or azide tags, chemical reporters, have been developed that enable fluorescent imaging and proteomic profiling of modified proteins using bioorthogonal ligation methods. For this presentation, I will summarize the currently available chemical reporters and highlight their utility to discover and quantify the roles of protein modifications in biology. Specifically, I will discuss recent efforts from my laboratory to explore the roles of protein S-palmitoylation in innate immunity.